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KMID : 1094720130180010065
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Biotechnology and Bioprocess Engineering
2013 Volume.18 No. 1 p.65 ~ p.74
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An alternative approach to the fermentation of sweet sorghum juice into biopolymer of poly-β-hydroxyalkanoates (PHAs) by newly isolated, Bacillus aryabhattai PKV01
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Tanamool Varavut
Imai Tsuyoshi
Danvirutai Paiboon
Kaewkannetra Pakawadee
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Abstract
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This work revealed for the first time the possible use of a newly isolated Bacillus aryabhattai PKV01 for poly-β-hydroxyalkanoates (PHAs) production from fermentative sweet sorghum juice. Its growth and PHA production were investigated under different pH and nitrogen sources. Medium composition was optimized using statistical tools. The highest biomass and PHA content were reached at pH 6.5 with the use of urea. Plackett-Burman design was then applied to test the relative importance of medium components and process variables on cell growth and PHA production. Cell growth and PHAs production were affected by total sugar and urea and were subjected to optimize the sorghum juice medium using response surface methodology (RSM) via central composite design (CCD). The predicted optimal culture composition was achieved. Maximum dry cell weight and PHAs were obtained using a flask and almost double the amount was achieved using a bioreactor. After PHA recovery, the structure and thermal properties were characterised and revealed to be similar to the standard of poly-β-hydroxybutyrate (PHB).
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KEYWORD
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sweet sorghum juice, Bacillus aryabhattai, biopolymer, PHAs, RSM
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